Fluorescence determination of Cu2+and glyphosate in blood samples based on modified carbon dots
Objective To establish a highly effective fluorescence method for the detection of copper ions and glyphosate in the blood samples by coupling 4-butyl-3-thiosemicarbazone(BTSC)with carbon dots(CDs).Methods The modified CDs(B-CDs)were prepared by modifying the surface of CDs with BTSC.Then,200 μl 0.1 g/L of B-CDs and copper ion solution were added into two 1.5 ml centrifuge tubes respectively.Subsequently,the solution was diluted to 1.0 ml using PBS buffer solution.After mixing and reacting for 5 min,the fluorescence intensity of the solution in the one centrifuge tube was scanned at an excitation wavelength of 340 nm.The content of copper ion was obtained according to the fluorescence intensity.Based on the fluorescence intensity change of the B-CDs/Cu2+syetem the content of glyphosate was gained after being added 50 μl glyphosate into another centrifuge tube,and reacted for 10 min.Results The linear relationship between the fluorescence intensity and concentration of copper ion was y=752.81-21.25x in the range of 0.2-30 μmol/L with the correlation coefficient of 0.998 1,the limit of detection was 5.05 nmol/L.The linear relationship of glyphosate was y=421.97+14.14x(r=0.998 9)with the detection limit of 0.27 μmol/L and the rates of recovery were 98.3％-105.1％,the RSDs were 0.8-4.1％.Conclusion This new fluorescence method for the determination of copper ion and glyphosate in blood samples sis has high accuracy and feasibility.
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