血液和尿液多种微量元素的电感耦合等离子质谱法
Determination of trace elements in blood and urine by inductively coupled plasma mass spectrometry
王玺凯 1王琳 1房怡君 1马景晶1
作者信息
- 1. 西安市中心医院职业病科,西安 710003
- 折叠
摘要
目的 建立人体血液和尿液中多种微量元素的电感耦合等离子质谱法.方法 采用1％硝酸+0.1％Triton X-100+2％异丙醇将血液或尿液样品直接稀释后进样,采用内插入模式校正干扰,用氦气碰撞模式分析样品中19种金属元素.结果 各元素在1～200 μg/L线性范围内,所得回归方程的线性关系良好,r＞0.999.该方法检出限为0.002～1.699 μg/L,定量下限为0.01～5.66 μg/L;血液、尿液的加标回收率分别为86.2％～115.6％和81.8％～105.6％,RSD＜6.3％.结论 该方法前处理操作简便、分析速度快、灵敏度高,可同时测定多种金属元素,适用于血液和尿液中19种元素的测定.
Abstract
Objective To establish a method for determination of trace elements in human blood and urine by inductively coupled plasma mass spectrometry.Methods The blood or urine samples were directly diluted by 1％nitric acid+0.1％Triton X-100+2％isopropanol and sample injection was conduced.The interferences were corrected by using the internal insertion mode,and 19 metallic elements in the samples were analyzed by using the helium collision mode.Results The linear ranges of the regression equations were 1-200 μg/L with the correlation coefficients greater than 0.9993.The limits of detection of the method were 0.002-1.699 μg/L,the lower limits of quantification of the method were 0.01-5.66 μg/L,the recoveries of the method in the blood ranged from 86.2％to 115.6％,and those in urine were 81.8％-105.6％,and the RSDs were less than 6.3％.Conclusion The method is simple in pre-treatment operation,fast in analysis speed and high in sensitivity,and can simultaneously determine a variety of metal elements,and is applicable to the determination of 19 elements in the blood and urine.
关键词
电感耦合等离子体质谱/全血/尿液Key words
Inductively coupled plasma mass spectrometry/Blood/Urine引用本文复制引用
基金项目
陕西省重点研发计划(2021SF-473)
西安市中心医院科研项目(2023YB01)
出版年
2024
NETL
NSTL
万方数据