Determination of trace elements in blood and urine by inductively coupled plasma mass spectrometry
Objective To establish a method for determination of trace elements in human blood and urine by inductively coupled plasma mass spectrometry.Methods The blood or urine samples were directly diluted by 1％nitric acid+0.1％Triton X-100+2％isopropanol and sample injection was conduced.The interferences were corrected by using the internal insertion mode,and 19 metallic elements in the samples were analyzed by using the helium collision mode.Results The linear ranges of the regression equations were 1-200 μg/L with the correlation coefficients greater than 0.9993.The limits of detection of the method were 0.002-1.699 μg/L,the lower limits of quantification of the method were 0.01-5.66 μg/L,the recoveries of the method in the blood ranged from 86.2％to 115.6％,and those in urine were 81.8％-105.6％,and the RSDs were less than 6.3％.Conclusion The method is simple in pre-treatment operation,fast in analysis speed and high in sensitivity,and can simultaneously determine a variety of metal elements,and is applicable to the determination of 19 elements in the blood and urine.
Inductively coupled plasma mass spectrometryBloodUrine
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