Determination of six kinds of plant growth regulators in fruits and vegetables by high performance liquid chromatography/electrospray tandem mass spectrometry
Objective To develop a test method for simultaneous determination of six plant growth regulators in fruits and vegetables by HPLC-MS/MS,including paclobutrazol,gibberellic acid,2,4-dichlorophenoxy acetic acid,thidizauron,forchlorfenuron and 4-chlorophenoxy acetic acid.Methods The samples were extracted by acetonitrile and combined with QuEChERS extraction kit,purified by C18 powder.Quantification of 6 plant growth regulators was achieved by HPLC-MS/MS with multiple reaction monitoring(MRM)using external standard method.Results Within the linear range of 0.1 to 100 ng/ml,the regression for the six target compounds exhibited good linearity with an r value greater than 0.994,indicating a good linear relationship within the selected concentration range.The limits of detection ranged from 0.01 to 0.05 μg/kg,and the quantification limits ranged from 0.05 to 0.25 μg/kg,respectively.The recoveries ranged from 70.4％to 110.8％,with relative standard deviations(RSDs)of 2.28％-8.53％.Conclusion This method is reliable,showing high sensitivity,good recovery and repeatability,and is applicable to rapid detection of six plant growth regulators in fruits and vegetables.
HPLC-MS/MSQuEChERS extractionPlant growth regulatorFruits and vegetables
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