果蔬中6种生长调节剂的高效液相色谱-串联质谱测定法
Determination of six kinds of plant growth regulators in fruits and vegetables by high performance liquid chromatography/electrospray tandem mass spectrometry
高晓明 1张续2
作者信息
- 1. 中国食品药品检定研究院,北京 102629
- 2. 中国疾病预防控制中心环境与人群健康重点实验室中国疾病预防控制中心环境与健康相关产品安全所
- 折叠
摘要
目的 建立同时测定常见水果蔬菜中多效唑、赤霉素、2,4二氯苯氧乙酸、噻苯隆、氯吡脲、4-氯苯氧乙酸等6种植物生长调节剂残留量的高效液相色谱-串联质谱(HPLC-MS/MS)方法.方法 样品采用乙腈联合QuEChERS提取包振荡提取,用C18粉末净化、多反应监测(MRM)、基质匹配标准溶液外标法定量.结果 在0.1～100 ng/ml线性范围内,所得6种植物生长调节剂的回归方程均呈良好的线性关系,r＞0.994,表明标准曲线在所选的浓度范围内的线性关系良好.该方法的检出限为0.01～0.05 μg/kg,定量下限为0.05～0.25 μg/kg;平均回收率为70.4％～110.8％,RSD为2.28％～8.53％.结论 该方法分析速度快,灵敏度高,回收率、重现性好,可有效用于水果和蔬菜中6种植物生长调节剂残留的快速检测.
Abstract
Objective To develop a test method for simultaneous determination of six plant growth regulators in fruits and vegetables by HPLC-MS/MS,including paclobutrazol,gibberellic acid,2,4-dichlorophenoxy acetic acid,thidizauron,forchlorfenuron and 4-chlorophenoxy acetic acid.Methods The samples were extracted by acetonitrile and combined with QuEChERS extraction kit,purified by C18 powder.Quantification of 6 plant growth regulators was achieved by HPLC-MS/MS with multiple reaction monitoring(MRM)using external standard method.Results Within the linear range of 0.1 to 100 ng/ml,the regression for the six target compounds exhibited good linearity with an r value greater than 0.994,indicating a good linear relationship within the selected concentration range.The limits of detection ranged from 0.01 to 0.05 μg/kg,and the quantification limits ranged from 0.05 to 0.25 μg/kg,respectively.The recoveries ranged from 70.4％to 110.8％,with relative standard deviations(RSDs)of 2.28％-8.53％.Conclusion This method is reliable,showing high sensitivity,good recovery and repeatability,and is applicable to rapid detection of six plant growth regulators in fruits and vegetables.
关键词
高效液相色谱-串联质谱/QuEChERS提取/植物生长调节剂/水果和蔬菜Key words
HPLC-MS/MS/QuEChERS extraction/Plant growth regulator/Fruits and vegetables引用本文复制引用
出版年
2024
NETL
NSTL
万方数据