The effect of heated cigarette smoke on the monolayer of pulmonary surfactant
Objective To explore the effect of heated cigarette smoke on pulmonary surfactant(PS)monolayer after it enters the lung,and to reveal that it may cause damage to lung function and health.Methods In this study,aqueous solution prepared by heating cigarette smoke and natural PS extracted from pig lungs were used as experimental materials to determine the changes of interfacial chemical properties such as surface tension,foaming properties and phase behavior of PS before and after exposure to heated cigarette smoke.Results Heating cigarette smoke will adsorb phospholipids and protein components in PS(P＜0.05),resulting in a decrease in phospholipids and protein content,which in turn increases surface tension and decreases foaming performance.At the maximum exposure,phospholipids and protein content decreased from 46.86 mg/L and 5.09 mg/L to 28.16 mg/L and 3.52 mg/L,surface tension increased from 27.37 mN/m to 37.76 mN/m,and foaming performance decreased from 48.56 ml to 34.57 ml.It was observed by the Brewster angle microscope that the flue gas components(particulate matter and organic matter)were also embedded and accumulated on the PS film,resulting in the outward expansion of the π-A isotherm of the PS film.Conclusion Exposure to heated cigarette smoke may affect the normal function of PS in the alveoli,which may pose a potential hazard to lung health.
万方数据
维普
