Effect of hypothermic and hypoxic pretreatment on acute radiation injuries in mice under simulate highland environment
Objective To understand the effect of hypothermia and hypoxia on acute radiation injuries and provide the experimental supports for the treatment of acute radiation injuries on the plateau.Methods The hypothermic and hypoxic equipment was prepared to develop a simulate the hypothermic and hypoxic environment at an altitude of 4,000 meters,and the experimental C57 mouse model of hypothermia and hypoxia combined with acute radiation injury was established.C57 male mice were randomly divided into the room temperature and normoxia control group,hypothermia plus hypoxia control group,room temperature and normoxia plus radiation group and hypothermia and hypoxia plus radiation group.The survival rate,routine blood indexes,count of nucleated cells in bone marrow and bone marrow histological changes were determined,and MDA and SOD contents in serum were detected at 6 h after radiation.Results Compared with the room temperature and normoxia plus radiation group,the survival rate of mice in the hypothermia and hypoxia plus radiation group was improved,the bone marrow injury was less severe,the bone marrow nucleated cells were more(P＜0.05),and the counts of white blood cells,lymphocytes and platelets in peripheral blood were higher at the 7th,14th and 21st days after radiation.Moreover,in serum,MDA content was lower(P＜0.01),and SOD content was higher(P＜0.01)at 6 h after radiation.Interestingly,compared with the room temperature and normoxia control group,the hypothermia and hypoxia control group had less white blood cells at day 7,14 and 21(P＜0.05),had less lymphocytes at day 3,7 and 14(P＜0.05),and had less blood platelet at day 7 after radiation(P＜0.05).Conclusion Hypothermic and hypoxic pretreatment may reduce acute radiation damage,whereas it also may have negative effects on routine peripheral blood indexes.
Hypothermia and HypoxiaRadiationBone marrowAntioxidase
万方数据
维普
