Distribution characteristics of b-lactam resistance bacteria and resistance genes in air during heavy pollution weather in urban Beijing
Objective To compare the differences in β-lactam antibiotic resistance among airborne bacteria under polluted and non-polluted weather conditions in Beijing.Methods Air samples were collected from urban areas of Beijing during polluted and non-polluted weather using a six-stage Andersen sampler and high-flow membrane filters.Environmental factors were recorded during sampling.Cultivable bacteria were isolated,and the 16S rRNA gene was amplified and sequenced for identification.Antibiotic resistance phenotypes were detected through antimicrobial susceptibility testing,and metagenomic sequencing was performed on enriched samples from membrane filter elution.Results Airborne bacterial abundance,species diversity,antibiotic resistance gene abundance,and β-lactam resistance gene abundance were all higher during polluted weather compared with non-polluted weather.The proportion of inhalable bacteria increased by more than 10％during severe pollution events.The abundance of Firmicutes was significantly elevated in polluted weather samples.A total of 134 antibiotic resistance genes(AROs)were identified,including 26 β-lactam resistance genes(19.40％).Humidity and temperature were significantly correlated with the abundance of multiple β-lactam resistance genes.Conclusion Attention should be paid to the issue of airborne bacteria developing resistance to β-lactam antibiotics.
万方数据
维普
