Determination of 16 elements in breast milk by graphite digestion,collision mode,inductively coupled plasma-mass spectrometry
Objective To develop a method for the determination of 16 elements in breast milk by graphite digestion,collision mode(KED),inductively coupled plasma-mass spectrometry(ICP-MS).Methods Breast milk was digested by using a graphite digester with 2.5 ml of nitric acid+0.5 ml of hydrogen peroxide as the digesting solution.The solution was digested at 150 ℃ for 45 min,and then the acid was driven off at 100 ℃ for 30 min to obtain a 0.5 ml solution,which was used to determine 16 elements in the samples by using the KED mode of the ICP-MS instrument,namely vanadium,chromium,manganese,cobalt,nickel,copper,zinc,arsenic,selenium,strontium,barium,molybdenum,cadmium,tin,antimony and lead.Results The linearity of the regression equations for the 16 elements was good in the 0.05-2 000 μg/L range,with r＞0.999.The limits of detection(LOD)of the method were in the range of 0.04-5 μg/L,and the lower limits of quantification(LOQ)were in the range of 0.1-15 μg/L.The coefficients of variation(CV)for each element were in the range of 0.5％-11.3％,and the recoveries were in the range of 81.6％-118％.Conclusion The method has a wide linear range,low detection limit,high sensitivity,good accuracy,safe operation,and it is applicable to the determination of 16 elements in breast milk.
万方数据
维普
