葛根总黄酮染毒对膀胱癌T24细胞株N-myc下游调控基因1 mRNA表达的影响
Effect of puerariae radix flavones on mRNA expression of N-myc downstream regulatory gene 1 in bladder cancer cell line T24
董晶 1郭毓鹏 1冀涛 1高青 2关红军 1牛莹莹 1马艳斌 3荣胜忠1
作者信息
- 1. 牡丹江医学院公共卫生学院,黑龙江牡丹江 157011
- 2. 牡丹江医学院临床实验中心
- 3. 哈尔滨理工大学理学院
- 折叠
摘要
目的 探讨葛根总黄酮(puerariae radix flavoues,PRF)对膀胱癌T24细胞株的作用机制,为以后临床治疗膀胱癌提供一个新的思路.方法 将T24细胞暴露于0(对照)、50、100、200 μg/m l的PRF溶液中培养24 h和48 h后,采用Annexin V-FITC/PI法检测细胞凋亡情况;采用qRT-PCR法检测N-myc下游调控基因1(NDRG-1)的表达.结果 与对照组比较,200 μg/ml PRF染毒24 h及100、200 μg/ml PRF染毒48 h时T24细胞的凋亡率均较高,差异有统计学意义(P＜0.05);与24 h比较,100、200 µg/ml PRF染毒48 h时T24细胞的凋亡率均较高,差异有统计学意义(P＜0.05);且随着PRF染毒时间的延长和染毒剂量的升高,T24细胞的凋亡率均呈上升趋势.与对照组比较,100、200 μg/ml PRF染毒24 h时T24细胞NDRG-1 mRNA的表达水平较高,差异均有统计学意义(P＜0.05);而各浓度PRF染毒48 h时T24细胞NDRG-1 mRNA的表达水平均无明显改变;与24 h比较,200 μg/ml PRF染毒48 h时T24细胞NDRG-1 mRNA的表达水平降低,差异均有统计学意义(P＜0.05).结论 PRF可诱导T24细胞凋亡,其机制可能是启动细胞外凋亡通路,激活下游调控基因NDRG-1高表达,进而参与膀胱癌T24细胞凋亡的调节.
Abstract
Objective To understand the effect of puerariae radix flavones on mRNA expression of N-myc downstream regulatory gene 1 in bladder cancer cell line T24 and provide a new evidence for future clinical treatment of bladder cancer.Methods After T24 cells were exposed to PRF at the doses of 0,50,100 and 200 μg/ml respectively for 24 h and 48 h,Annexin V-FITC/PI stained flow cytometry was used to detect apoptosis.The expression of N-myc downstream regulated gene 1(NDRG-1)wwas examined by qRT-PCR.Results Compared with the control group,the apoptosis rate of T24 cells was higher after 200 μg/ml PRF for 24 h and after 100 and 200 μg/ml PRF for 48 h,with significant difference(P＜0.05).Compared with 24 h,the apoptosis rate of T24 cells was higher after 100 and 200 μg/ml PRF for 48 h,the difference was statistically significant(P＜0.05).The apoptosis rate of T24 cells showed an increasing trend with the increase of PRF exposure time and dose.Compared with the control group,the expression level of NDRG-1 mRNA in T24 cells after 100 and 200 μg/ml PRF exposure for 24 h was higher,with significant differences(P＜0.05).There was no significant change in the expression of NDRG-1 mRNA in T24 cells after 48 h of PRF exposure.Compared with 24 h,the expression level of NDRG-1 mRNA in T24 cells significantly decreased after 200 μg/ml PRF exposure for 48 h(P＜0.05).Conclusion PRF may induce apoptosis in T24 cells,with a dose-time dependent manner.The mechanism may be to initiate the extrinsic apoptosis pathway,activate downstream regulatory gene NDRG-1,and then participate in the regulation of T24 bladder cancer cell apoptosis.
关键词
膀胱癌/人膀胱移行细胞癌细胞/葛根总黄酮/细胞凋亡/N-myc下游调控基因1Key words
Bladder cancer/T24 cells/Puerariae radix flavoues/Apoptosis/NDRG-1引用本文复制引用
出版年
2024
NETL
NSTL
万方数据