Effect of puerariae radix flavones on mRNA expression of N-myc downstream regulatory gene 1 in bladder cancer cell line T24
Objective To understand the effect of puerariae radix flavones on mRNA expression of N-myc downstream regulatory gene 1 in bladder cancer cell line T24 and provide a new evidence for future clinical treatment of bladder cancer.Methods After T24 cells were exposed to PRF at the doses of 0,50,100 and 200 μg/ml respectively for 24 h and 48 h,Annexin V-FITC/PI stained flow cytometry was used to detect apoptosis.The expression of N-myc downstream regulated gene 1(NDRG-1)wwas examined by qRT-PCR.Results Compared with the control group,the apoptosis rate of T24 cells was higher after 200 μg/ml PRF for 24 h and after 100 and 200 μg/ml PRF for 48 h,with significant difference(P＜0.05).Compared with 24 h,the apoptosis rate of T24 cells was higher after 100 and 200 μg/ml PRF for 48 h,the difference was statistically significant(P＜0.05).The apoptosis rate of T24 cells showed an increasing trend with the increase of PRF exposure time and dose.Compared with the control group,the expression level of NDRG-1 mRNA in T24 cells after 100 and 200 μg/ml PRF exposure for 24 h was higher,with significant differences(P＜0.05).There was no significant change in the expression of NDRG-1 mRNA in T24 cells after 48 h of PRF exposure.Compared with 24 h,the expression level of NDRG-1 mRNA in T24 cells significantly decreased after 200 μg/ml PRF exposure for 48 h(P＜0.05).Conclusion PRF may induce apoptosis in T24 cells,with a dose-time dependent manner.The mechanism may be to initiate the extrinsic apoptosis pathway,activate downstream regulatory gene NDRG-1,and then participate in the regulation of T24 bladder cancer cell apoptosis.
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