目的 分析细胞周期蛋白D1(CCND1)、神经轴突导向分子5A(SEMA5A)基因多态性与幽门螺杆菌(HP)感染相关性胃癌的关系.方法 选定杭州市富阳区第二人民医院消化内科2022年2月至2023年12月就诊的80例胃癌患者设为胃癌组,以及同期80例慢性胃炎患者设为对照组,检测CCND1、SEMA5A基因多态性,比较两组CCND1、SEMA5A基因频率分布,对HP感染及CCND1、SEMA5A基因多态性与胃癌的关系进行logistic回归分析,采用Spearman相关分析研究CCND1、SEMA5A基因多态性与胃癌易感性的相关性.结果 胃癌组CCND1基因GG、GA、AA 及SEMA5A基因TT、TC、CC与对照组比较,差异有统计学意义(P＜0.05).在HP感染阳性的52例患者中,胃癌组CCND1基因GG频率(5.77％)、AA 基因型频率(71.15％)以及 G 频率(17.31％)、A 等位基因频率(82.69％)与对照组(44.00％、20.00％、62.00％、38.00％)比较,差异有统计学意义(P＜0.05).胃癌组SEMA5A基因TT(48.08％)、CC基因型频率(25.00％)以及T(61.54％)、C等位基因频率(38.46％)与对照组(8.00％、40.00％、28.00％、72.00％)比较,差异有统计学意义(P＜0.05).HP感染阴性的28例患者中,胃癌组CCND1及SEMA5A的基因型频率、等位基因频率与对照组比较,差异无统计学意义(P＞0.05).CCND1、SEMA5A基因多态性与胃癌易感性存在明显相关性(r=0.624、0.603,均P＜0.05).结论 CCND1、SEMA5A基因多态性与HP感染相关性胃癌的发生联系密切,HP感染阳性CCND1基因AA、SEMA5A基因TT携带者胃癌易感性会增加.
Relationship between CCND1 and SEMA5A gene polymorphisms and gastric cancer associated with helicobacter pylori infection
Objective To understand the relationship between gene polymorphisms of cyclin D1(CCND1)and axon-directing molecule 5A(SEMA5A)and gastric cancer associated with helicobacter pylori(HP)infection.Methods Totally 80 patients with gastric cancer who visited the Department of Gastroenterology at the Second People's Hospital of Fuyang District,Hangzhou from February 2022 to December 2023 were chosen as the gastric cancer group,and 80 patients with chronic gastritis during the same period were chosen as the control group.CCND1 and SEMA5A gene polymorphisms were detected,and the frequency distribution of CCND1 and SEMA 5A genes was compared between the two groups.Logistic regression analysis was conducted to analyze the relationship between HP infection,CCND1 and SEMA5A gene polymorphisms and gastric cancer susceptibility.Spearman analysis was used to analyze the correlation between CCND1 and SEMA5A gene polymorphisms and gastric cancer susceptibility.Results The differences in CCND1 gene GG,GA,AA,SEMA5A gene TT,TC,CC between the gastric cancer group and the control group were statistically significant(P＜0.05).Among the 52 patients with positive HP infection,the CCND1 gene GG frequency(5.77％),AA genotype frequency(71.15％),G frequency(17.31％),and A allele frequency(82.69％)in the gastric cancer group were significantly different from those in the control group(44.00％,20.00％,62.00％,38.00％)(P＜0.05).The SEMA5A gene TT(48.08％),CC genotype frequency(25.00％),T(61.54％),and C allele frequency(38.46％)in the gastric cancer group were significantly different from those in the control group(8.00％,40.00％,28.00％,72.00％)(P＜0.05).Among the 28 patients with negative HP infection,there was no statistically significant difference in genotype frequency and allele frequency of CCND1 and SEMA5A between the gastric cancer group and the control group(P＞0.05).There was a significant correlation between CCND1,SEMA5A gene polymorphism and susceptibility to gastric cancer(r=0.624,0.603,P=0.016,0.013).Conclusion CCND1 and SEMA5A gene polymorphisms are closely associated with HP-infected gastric cancer,and HP-infected CCND1 gene AA and SEMA5A gene TT carriers are more susceptible to gastric cancer.
Cyclin D1Axon-directing molecule 5AGene polymorphismHelicobacter pyloriGastric cancer
NETL
NSTL
万方数据
