Toxic effects of dioctyl phthalate on Saccharomyces cerevisiae BY4742 cells
Objective To understand the toxicity and mechanism of dioctyl phthalate to saccharomyces cerevisiae BY4742 cells.was studied by using different concentrations of dioctyl phthalate on the growth,skeleton and apoptosis of yeast cells.Methods The cells of saccharomyces cerevisiae BY4742 were exposed to dioctyl phthalate at the doses of 0.00 mg/L,1.00 mg/L,10.0 mg/L and 100.0 mg/L respectively,and the growth of the cells was observed.The cells of saccharomyces cerevisiae BY4742 were exposed to dioctyl phthalate at the doses of 0.00 mg/L,0.10 mg/L,0.25 mg/L and 0.50 mg/L respectively,and the cytoskeleton,nucleus and apoptosis of the cells were observed by staining,and the expression of cytoskeletons-related genes was detected by qRT-PCR.Results Dioctyl phthalate had no obvious effect on the growth of saccharomyces cerevisiae BY4742 cells at a low concentration of 1.00 mg/L(P＞0.05),that is,no obvious acute toxic effect,but had obvious inhibitory effect on the growth of saccharomyces cerevisiae BY4742 cells at a high concentration of 10.0 mg/L and 100.0 mg/L(P＞0.05).After exposure to dioctyl phthalate at low concentrations of 0.10 mg/L,0.25 mg/L,and 0.50 mg/L,BY4742 saw nuclear fragmentation,the formation of nuclear fragments with different size patches,the cytoskeleton showed spots and plaques,and the number of apoptosis increased.The transcription levels of cytoskeleton-related proteins Arc15p,Las17p and Crn1p were significantly down-regulated by qRT-PCR compared with the control group(P＜0.05).With the increase of dioctyl phthalate,the skeletal abnormality rate of BY4742 cells increased,and the relative transcription levels of protein genes Arc15,Las17 and Cm1 in the skeletal of the cells were significantly down-regulated.Conclusion Dioctyl phthalate has toxic effect on BY4742 cells,and the mechanism may be related to the destruction of the nuclear structure of cells and the alteration of gene transcription levels of cytoskeletal proteins.
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