Correlation analysis of T lymphocyte subset and EBV infection in patients with allogeneic hematopoietic stem cell transplantation
Objective To analyze the correlation between T lymphocyte subset and Epstein-Barr virus(EBV)infection after allogeneic hematopoietic stem cell transplantation(allo-HSCT).Methods Totally 67 patients who underwent allo-HSCT in the General Hospital of Northern Theater Command from May 2015 to December 2021 were selected as research group,and 54 hematopoietic stem cell transplant donors were recruited as control group.The EBV-DNA content was detected by real-time fluorescent quantitative PCR.The level of T lymphocyte subset was detected by flow cytometry,and the differences in the distribution levels of T lymphocyte subsets was compared between the 2 groups.The correlation between the EBV-DNA load and the level of T lymphocyte subset was analyzed.Results The EBV-DNA positive rate and copy number of the patients with allo-HSCT were all higher than those in the control group(P<0.05),and CD3+CD4+T lymphocyte and CD4+/CD8+ in the allo-HSCT patients with positive EBV-DNA were significantly lower than those in the control group(P=0.001 and P=0.005).The percentages of CD3+CD4+ T lymphocyte in EBV negative group and EBV positive group in the research group were mainly distributed in 20% -<40% ,the difference between the 2 groups was statistically significant(χ2=9.269,P=0.010).There was no significant different in the percentage distribution of CD3+CD8+ T lymphocytes(χ2=0.279,P=0.870).The CD4+/CD8+ ratio of EBV positive group in the research group was mainly distributed in the range of 0.6-<1.2,and the difference between the 2 groups was statistically significant.The viral load of EBV-DNA was positively correlated with CD3+CD8+ cells(P<0.01)and was negatively correlated with the ratio of CD4+/CD8+(P<0.01).Conclusion The active EBV infection is closely related to the T lymphocyte subset in the allo-HSCT patients,and the detection of peripheral T lymphocyte subset is of great significance for assessing the severity of the disease in the allo-HSCT patients with EBV infection.
NETL
NSTL
万方数据
维普
