Determination of Baicalin in Chaiguijiebiao granule by high performance liquid chromatography
Objective To establish a method for the determination of baicalin in Chaiguijiebiao granule by high performance liquid chromatography(HPLC)and further improve the quality standards of Chaiguijiebiao granule.Methods The sample of Chaiguijiebiao granule was treated by ultrasound to prepare the test product solution.Specificity test,linear relationship test,precision test,repeatability test,and sample recovery test were investigated by HPLC.On the basis of qualified methodology,the content of baicalin in 3 batches of Chaiguijiebiao granule was determined.Results The concentration of baicalin in the 3 batches of samples was 4.07 mg/g,4.11 mg/g and 4.40 mg/g,respectively.Preliminary quality standard of Chaiguijiebiao granule was established,with baicalin content ranging from 4.0 to 4.5 mg/g.Conclusion The HPLC method is simple,accurate and reliable,and can be used for the determination of baicalin in Chaiguijiebiao granule.
NETL
NSTL
万方数据
维普
