口面部疼痛模型小鼠前扣带回皮质神经元电生理特性研究
Electrophysiological characteristics of anterior cingulate cortex neurons in mice with orofacial pain
郭梓琰 1陈佳楠 1董蕾 1高天 1张新海1
作者信息
- 1. 200433 上海,海军军医大学第一附属医院口腔科
- 折叠
摘要
目的 观察完全弗氏佐剂(complete Freund's adjuvant,CFA)诱导的口面部疼痛模型小鼠大脑前扣带回皮质(an-terior cingulate cortex,ACC)神经元电生理特性.方法 将 18 只小鼠随机分为Sham组和CFA组,CFA组小鼠右侧面部胡须垫下注射CFA,构建口面部疼痛模型.Sham组小鼠注射生理盐水.用Von-Frey细丝检测口面部机械性疼痛阈值,采用膜片钳方法观察Sham组和CFA组小鼠ACC神经元兴奋性突触后电流(excitatory postsynaptic current,EPSC)和动作电位(action potential,AP)的发放情况.结果 与Sham组相比,CFA组小鼠面部胡须垫下注射CFA后诱发机械性疼痛阈值较低(P<0.05).CFA组小鼠ACC脑区神经元的EPSC的频率和幅值均高于Sham组(P<0.05).当刺激电流在 100、125、150、175、200 pA时,CFA组小鼠ACC脑区神经元的AP发放次数高于Sham组(P<0.05).结论 ACC脑区神经元活性在口面部疼痛的发生与发展中呈现增加的趋势,可能与口面部疼痛的形成密切相关.
Abstract
Objective To observe the electrophysiological characteristics of anterior cingulate cortex(ACC)neurons in mice with orofacial pain induced by complete Freund's adjuvant(CFA).Methods Eighteen mice were randomly divided into Sham group and CFA group.CFA was injected under the right facial beard pad in mice to construct a model of orofacial pain.Normal saline was injected in the Sham group.Von-Frey filament was used to detect the threshold of mechanical orofacial pain.The excitatory postsynaptic current(EPSC)and action potential(AP)of ACC neurons were observed in the Sham group and CFA group by patch clamp method.Results The threshold of mechanical pain in the CFA group was significantly lower than that in the Sham group after injection(P<0.05).The frequency and amplitude of the EPSC of ACC neurons in the CFA group were significantly higher than those in the Sham group(P<0.05).AP results showed that when the stimulus current was 100,125,150,175 and 200 pA,the discharge frequency of ACC neurons in the CFA group was significantly higher than that in the Sham group(P<0.05).Conclusion The activity of ACC neurons increases in the development and prgression of orofacial pain in mice and may be closely related to the formation of orofacial pain.
关键词
口面部痛/电生理/前扣带回皮质/兴奋性突触后电流/动作电位Key words
Orofacial pain/Electrophysiology/Anterior cingulate cortex/Excitatory postsynaptic current/Action potential引用本文复制引用
出版年
2024
NETL
NSTL
万方数据