Expression and regulatory network of autophagy-related genes in mesenchymal stem cells from bone marrow of primary senile osteoporosis
Objective To study the expression levels of autophagy-related genes(ARGs)in mesenchymal stem cells from bone marrow of primary senile OP,and to explore its regulatory network in the genome based on the genome chip data.Methods Differentially expressed genes(DEGs)of mesenchymal stem cells from bone marrow of primary senile OP were screened from GSE35959.In combination with the human autophagy database(HADb),differentially expressed-autophagy related genes(AR-DEGs)in bone marrow mesenchymal stem cells of senile OP were screened.Then STRING 11.0 database was used to establish a protein-protein interaction(PPI)network.Transcription factor(TF)-gene-miRNA co-expression network and environmental chemicals-AR-DEGs regulatory network were established based on Network Analyst.Results A total of 2 866 DEGs were involved in the development and progression of primary OP in the elderly,including 2 592(90.44%)up-regulated genes and 274(9.56%)down-regulated genes.Thirty-one AR-DEGs related to primary senile OP were obtained,and they were all highly expressed.Tumor protein 53(TP53),epidermal growth factor receptor(EGFR),ubiquitin C(UBC),and cyclin deperdent kinase inhibitor 1A(CDKN1A)showed important biological effects on primary senile OP.The autophagy was deeply affected by TFs,miRNAs,and environmental factors in primary senile OP.Conclusion The overexpression of autophagy is involved in the development and progression of primary senile OP.This process is related to immune function and is affected by a variety of TFs,microRNAs,trace elements and environmental chemicals.But the regulation mechanism still needs in-depth study.
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