Preparation of Glycerol Phosphatidylcholine by Immobilized Enzyme
Immobilized lipase has the advantages of safe green,strong specificity and easy separation.In order to study the preparation of glycerol phosphorylcholine(Glycerol phosphorylcholine,GPC)by enzymatic hydrolysis of soybean phospholipids in organic phase,taking the relative content of GPC as the evaluation index,the effects of reaction time,substrate concentration,water addition and enzyme addition on the degree of phospholipid hydrolysis were investigated.On the basis of single factor test and response surface test,the optimum process conditions for preparing GPC were as follows:reaction time 37.7 h,substrate concentration 16%,water addition 63%,enzyme addition 17%.Under these conditions,the relative content of GPC was 78%.After further purification by silica gel column chromatography,the relative content of GPC could reach 99.8%.Experiments showed that this method could effectively prepare high purity GPC.
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