Taking British red kidney bean as raw material,the extraction process was optimized by response surface experiment,and the antioxidant activity of British red kidney bean polysaccharide was analyzed in vitro.The results showed that the optimal extraction process of polysaccharide from British red kidney bean was as follows:extraction temperature 90℃,extraction time 1.5 h,solid-liquid ratio 1∶40 g·mL-1,and the optimal extraction rate of polysaccharide was 4.86%.The antioxidant activity test showed that the antioxidant activity of British red kidney bean polysaccharide was dose-effect dependent on its concentration.When the concentration of polysaccharide was 5 mg·mL-1,the scavenging rates of DPPH free radical,·OH free radical and O2-free radical were 69.85%,53.61%and 21.36%,respectively.
British red kidney beanpolysaccharideresponse surface methodologyextractionantioxidant activities