首页|连续秸秆还田条件下氮肥减施对稻田土壤真菌多样性的影响

连续秸秆还田条件下氮肥减施对稻田土壤真菌多样性的影响

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为探究在连续全量秸秆还田条件下,氮肥减施对土壤真菌多样性的影响,在黑龙江省前进农场科技示范区进行了七年的定位试验,试验共设 7 个处理,分别为无秸秆,不施氮肥(N0S0);单施秸秆(N0S);秸秆还田+100%氮肥(NS);秸秆还田+氮肥减施 10%(N1S);秸秆还田+氮肥减施 20%(N2S);秸秆还田+氮肥减施 30%(N3S);以不施秸秆常规施氮肥(NS0)为对照,比较 0~20 cm土层土壤微生物多样性变化.结果表明:秸秆还田条件下氮肥减施可显著提高腐生菌和植物寄生物相对丰度,增加子囊菌门(Ascomycota)、担子菌门(Basidiomycota)、被胞霉门,unclassified_Fungi等真菌水平相对丰度;氮肥减施 20%(N2S)与氮肥减施 30%(N3S)对增加真菌OTU值,真菌ACE指数和Chao1 指数,增强腐生菌作用尤为显著.
Effects of Nitrogen Fertilizer Reduction on Soil Fungal Diversity in Paddy Field under Continuous Straw Returning
In order to explore the effect of nitrogen fertilizer reduction on soil fungal diversity under the condition of continuous full-amount straw return,a seven-year positioning experiment was carried out in the science and technology demonstration zone of Qianjin farm in Heilongjiang province,and a total of 7 treatments were set up,with no straw and no nitrogen fertilizer(N0S0).Single straw(N0S);Straw return +100%nitrogen fertilizer(NS);Straw return + nitrogen fertilizer reduction by 10%(N1S);Straw return+ nitrogen fertilizer reduction by 20%(N2S),straw return+nitrogen fertilizer application reduction by 10%(N3S).The changes of soil microbial diversity in 0-20 cm soil layer were compared by conventional nitrogen fertilizer(NS0)without straw application.The results showed that the reduction of nitrogen fertilizer application under the condition of straw return could significantly increase the relative abundance of saprophytes,soil saprophytes and plant parasites,and increase the relative abundance of fungi such as Ascomycota,Basidiomycota,Angiomycota,and unclassified_Fungi.Nitrogen fertilizer reduction of 20%(N2S)and nitrogen fertilizer reduction of 30%(N3S)increased fungal OUT value,fungal ACE index and Chao1 index,and enhanced saprophytes.

straw returnnitrogen fertilizer reductionmicrobial diversity

周榆越、焦峰

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黑龙江八一农垦大学农学院,大庆 163319

秸秆还田 氮肥减施 高通量测序 微生物多样性

2024

黑龙江八一农垦大学学报
黑龙江八一农垦大学

黑龙江八一农垦大学学报

影响因子:0.888
ISSN:1002-2090
年,卷(期):2024.36(1)
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