The aim of this experiment was to investigate the effect of different concentrations of formononetin combined with tyrosine and cysteine additions on the liver thermogenic capacity of mice in a low temperature environment,the mice were gavaged for 28 days after one week of pre-feeding using the different additions prior to hypothermic stimulation.The normothermic control group was kept at 26±2℃ every day,and the cold-exposed group was cold-stimulated in an artificial climate chamber at 4℃ for 3 h every day,and liver tissues were collected after four weeks.Liver tissue morphology was assessed by HE staining;the expression of UCP1 protein in liver tissues was quantified by immunohistochemistry;the expression levels of thermogenic factors PGC-1α,PPAR-γ and UCP1 in liver tissues were detected by Western Blot method.The results showed that tyrosine and cysteine combined with formononetin significantly alleviated cold-induced inflammatory cell infiltration and cell necrosis in mouse hepatocytes,and the expression levels of PGC-1α,PPAR-γ and UCP1 in liver were significantly up-regulated by the combination of the three factors,which in turn improved the thermogenic capacity of mouse liver.
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