Mechanism of Tyrosine and Cysteine Combined with Formononetin in Regulating Liver Thermogenesis in Mice under Low Temperature
The aim of this experiment was to investigate the effect of different concentrations of formononetin combined with tyrosine and cysteine additions on the liver thermogenic capacity of mice in a low temperature environment,the mice were gavaged for 28 days after one week of pre-feeding using the different additions prior to hypothermic stimulation.The normothermic control group was kept at 26±2℃ every day,and the cold-exposed group was cold-stimulated in an artificial climate chamber at 4℃ for 3 h every day,and liver tissues were collected after four weeks.Liver tissue morphology was assessed by HE staining;the expression of UCP1 protein in liver tissues was quantified by immunohistochemistry;the expression levels of thermogenic factors PGC-1α,PPAR-γ and UCP1 in liver tissues were detected by Western Blot method.The results showed that tyrosine and cysteine combined with formononetin significantly alleviated cold-induced inflammatory cell infiltration and cell necrosis in mouse hepatocytes,and the expression levels of PGC-1α,PPAR-γ and UCP1 in liver were significantly up-regulated by the combination of the three factors,which in turn improved the thermogenic capacity of mouse liver.
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维普
