Study on the Activity of N-dodecyl-2-(piridin-1-ium)Acetamide to Kill or Inhibit Pathogens
Quaternary ammonium surfactants are a kind of low toxicity and high efficiency broad-spectrum disinfectants,which can kill a variety of bacteria and viruses,and have broad application prospects in the disinfection of breeding industry and medical fields.To study the antibacterial and antiviral activity of N-dodecyl-2-(piridin-1-ium)acetamid(C12cmpC),a self-synthesized quaternary ammonium surfactant,against avian pathogens and bovine viral diarrhea virus(BVDV).The efficacy of C12cmpC in killing or inhibiting gram-negative and gram-positive bacteria in vitro was determined by MIC,MBC and time bactericidal tests.The antiviral activity of C12cmpC was preliminarily determined by cell morphology observation,cell activity detection and qRT-PCR detection of BVDV mRNA.The results showed that the MIC and MBC of C12cmpC against Escherichia coli(QS195313)were 250 mg·L-1 and 500 mg·L-1 respectively.The MIC and MBC of C12cmpC against Escherichia coli and Staphylococcus aureus were 31.25 mg·L-1 and 62.5 mg·L-1 respectively.In addition,the maximum safe concentration of C12cmpC for MDBK cells was 5 mg·L-1.The viral mRNA levels in MDBK cells were significantly decreased after 1,5,30 and 60 min treatment with BVDV.The results show that the new quaternary ammonium surfactant N-dodecyl-2-(piridin-1-ium)acetamide has good antibacterial and antiviral activity.This study provides a theoretical basis for the development of new disinfectants.
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维普
