Fine Mapping and Candidate Gene Analysis of Single Fruit Weight Gene in Melon
The light fruit type melon 1244(single fruit weight 22.59 g)was crossed with the heavy fruit type melon MS-5(single fruit weight 814.97 g)and the segregation population F2∶3 was obtained.Insertion-deletion(InDel)marker were used for fine mapping of a major QTL locus sfw2.2,which control single fruit weight,and the annotated genes in the mapping region were further analyzed to identify candidate target gene.Results showed,a major QTL locus sfw2.2 of single fruit weight was delimited between markers CY InDel11and CY InDel16,in which contained three known functional genes.Among of them,the relative expression of MELO3C029669 gene,which was fibrin synthesis-related gene,showed significant difference between two parents,suggesting that MELO3C029669 may be a candidate gene for controlling single fruit weight of melon.According to the gene structure differences between two parents of MELO3C029669,specific InDel molecular marker BCYInDel was designed to detect the signal fruit weight trait of 30 melon materials,and accuracy of results reached high accuracy 90.0%.
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