Effects of CUMS-induced Depression on Hippocampal Neurons and Inflammatory Pathways in Mice
To explore the effects of chronic unpredictability stress(CUMS)induced depression on hippocampal neurons and inflammatory pathways in mice,twelve C57BL/6 male mice were selected and divided into two groups(n=6):control group and CUMS group,and chronic unpredictable stress model was established.Two groups of mice were tested for sugar water preference and forced swimming.Nissner staining was used to observe the changes of neurons in the hippocampus of mouse brain.Western blot and qRT-PCR were used to detect the expression levels of inflammatory factors(TNF-α、IL-1β、iNOS and IL-6)in the hippocampus of mice.Compared with the control group,the sugar water preference index of mice in CUMS group decreased,but the rest time in water increased.The number of Nis-stained positive cells in CA1,CA3 and DG in the hippocampus of CUMS group was significantly decreased,and the protein and mRNA expressions of inflammatory factors(TNF-α,IL-1β,iNOS and IL-17)in the hippocampus were significantly increased.CUMS-induced depression could reduce the number of hippocampal neurons and promote neuroinfla-mmation in mice.
chronic unpredictable stressdepressionthe sea horseneureinflammation of nerves
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