槲皮素对断奶羔羊血清和肠道抗氧化指标及肠道屏障功能的影响
Effects of Quercetin on Serum and Intestinal Antioxidant Indices and Intestinal Barrier Function in Weaned Lambs
赵芳芳 1何文浩 1郭迪 1王文军 1张爱忠1
作者信息
- 1. 黑龙江八一农垦大学动物科技学院,大庆 163319
- 折叠
摘要
为探究槲皮素对断奶羔羊血清和肠道抗氧化指标及肠道屏障功能相关基因表达的影响,试验选取 12 只平均体重为(7.17±0.34)kg的 21 日龄健康断奶羔羊,随机分为 2 组,分别饲喂添加 0(对照组)和 100 mg·kg-1 BW槲皮素(槲皮素组)的日粮,试验期共21 d.结果表明:对照组羔羊血清中谷胱甘肽过氧化物酶(GSH-Px)活性显著低于槲皮素组(P＜0.05);2 组间血清总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性无显著差异(P＜0.05);对照组羔羊空肠组织GSH-Px和SOD活性显著低于槲皮素组(P＜0.05),MDA含量显著高于槲皮素组(P＜0.05),回肠组织MDA含量显著高于槲皮素组(P＜0.05);对照组羔羊空肠组织Occludin的相对表达量显著低于槲皮素组(P＜0.05).综合分析可知,饲粮中添加 100 mg·kg-1 BW槲皮素改善了断奶羔羊机体抗氧化能力和肠道屏障功能.
Abstract
To explore the effects of quercetin on serum and intestinal antioxidant indices and intestinal barrier function in weaned lambs,twelve weaned lambs with body weight of(7.17±0.34)kg were randomly assigned to two groups.Lambs in 2 groups were fed basal diets supplemented with 0(Con group)and 100 mg·kg-1 BW quercetin(Que group),respectively.The experimental period lasted for 21 days.The results showed as follows:the serum glutathione peroxidase(GSH-Px)activity in the Con group of lambs was significantly lower than that in the Que group(P＜0.05).There was no significant difference in serum total antioxidant capacity(T-AOC),superoxide dismutase(SOD)activity,catalase(CAT)activity(P＜0.05),and malondialdehyde(MDA)content between the two groups(P>0.05).The activities of GSH-Px and SOD in the jejunal tissue of lambs in the Con group were significantly lower than those in the Que group(P＜0.05),and the MDA content was significantly higher than that in the Que group(P＜0.05).The MDA content in the ileum tissue was significantly higher than that in the Que group(P＜0.05).The relative expression level of Occludin in jejunal tissue in Con group was significantly lower than that in the Que group(P＜0.05).These results indicated that quercetin at a dose of 100 mg·kg-1 BW could improve antioxidant capacity and intestinal barrier function of weaned lambs.
关键词
槲皮素/抗氧化/肠道屏障功能/羔羊Key words
quercetin/antioxidant indices/intestinal barrier function/lambs引用本文复制引用
基金项目
黑龙江省自然科学基金(LH2022C069)
黑龙江八一农垦大学学成引进人才科研启动计划(XYB202110)
出版年
2024
国家科技期刊平台
NSTL
万方数据