Propionic Acid Controls the Cell Proliferation and Differentiation in Bovine Pre-adipocytes
The proliferation and differentiation of preadipocytes is very important for normal adipose tissue development in dairy cows.In order to investigate the effect of propionic acid on the proliferation and differentiation of bovine preadipocytes at the cellular level,the experiment was divided into two groups.Experimental group:bovine preadipocytes were treated with 0.1～3 mmol·L-1 PA for 12～48 h,while control group was not treated.The lipid production of bovine preadipocytes was detected by oil red O staining,the proliferation of adipocytes was detected by EdU cell proliferation detection kit,and the cell cycle progression was analyzed by flow cytometry.Western Blot was used to detect the protein expression of several proliferation-related proteins and differentiation-related indicators in adipocytes.Including cyclin(CCND1,CCND2,CCND3,CCNE1),cyclin kinases(CDK2,CDK4,CDK6),peroxisome proliferator-activated receptor γ(PPARγ),CCAAT/enhancer binding protein α(C/EBPα),and sterol regulatory element binding transcription factor 1(SREBF1).The results showed that 0.1 mmol·L-1 PA treatment for 48 h promoted the proliferation of bovine preadipocytes,increased the proportion of cells in S phase and increased the protein abundance of cyclins compared with the untreated group.In addition,PA could promote the differentiation of bovine preadipocytes by up-regulating the protein expression of differentiation-related markers.These results indicated that 0.1 mmol·L-1 PA treatment for 48 h could promote the proliferation and differentiation of bovine preadipocytes and had a potential role in the development of normal adipose tissue.
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