Establishment of Inflammatory Injury Model of Intestinal Porcine Epithelial Cells Induced by Lipopolysaccharide
LPS-induced inflammatory injury in IPEC-J2 cells simulated early weaning stress in piglets in vivo,and built an inflammatory injury model of IPEC-J2 cells.Firstly,IPEC-J2 cells were treated with LPS of 0,0.1,1,10 and 100 μg·mL-1.MTS method was used to detect the proliferation rate of IPEC-J2 cells treated with LPS for 0,4,8,12,16 and 24 h.It was preliminarily determined that the appropriate concentration for the establishment of inflammatory injury model was 10 μg·mL-1,and the appropriate time was 16 h.In order to further determine the optimal concentration of LPS,IPEC-J2 cells were treated with LPS at concentrations of 0,0.1,1,10 and 100 μg·mL-1 for 16 hours to explore the effects of different concentrations of LPS on IPEC-J2 cells.The results showed that 0.1～100 μg·mL-1 LPS increased the contents or genes of IL-1β,IL-6,IL-8 and TNF-α,down-regulate the expression of ZO-1,occludin,and claudin-1 genes,and decreased the contents of IL-10,IgM,IgA and IgG in IPEC-J2 cells to varying degrees.The result of 10 μg·mL-1 LPS played a better effect.0.1～100 μg·mL-1 LPS increased the ROS content and apoptosis rate in IPEC-J2 cells to varying degrees,the result of 10 μg·mL-1 LPS played a better effect.On the basis of not affecting subsequent experiments,the result of 10 μg·mL-1 played a better effect.It was concluded that the appropriate conditions for LPS induced cells to construct an inflammatory injury model were 10 μg·mL-1 and 16 h.
pigletsIPEC-J2 cellslipopolysaccharideinflammatory injury model
万方数据
维普
