Preparation of Portulaca Oleracea L.Polysaccharide Liposomes by Reverse Evaporation and Its Immune-enhancing Effect Extrusion
The process of preparing purslane polysaccharide liposomes was optimized by a single-factor experiment,and its immunological enhancement was investigated.The three factors of soybean phosphatide concentration,soybean phosphatide to Tween-80 ratio and Ultrasound time were changed separately.Other conditions remained unchanged,and the encapsulation rate was the index.The toxicity and proliferative activity of mouse splenic lymph and macrophage Raw264.7 cells were tested to observe their immune effects.The optimal process conditions were soybean phosphatide concentration of 5 mg·mL-1,Tween 80 dosage of 15∶1,and Ultrasound time of 5 min,under which the encapsulation rate of purslane polysaccharide liposomes was 37.83%±1.49%,and the appearance of liposomes was transparent and clear with light blue opalescence.The maximum safe concentrations were 63 μg·mL-1.Compared with purslane polysaccharide liposomes and blank liposomes,the immune-enhancing effect of purslane polysaccharides and Raw264.7 macrophages was more significant.
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