To explore the baicalin(BA)on acute liver injury induced by acetaminoPhen(APAP)protection,and preliminarily discuss its mechanism,the mice were randomly divided into blank control group(CON group),baicalin control group(BA group),APAP model control group(APAP group),baicalin low-dose group(APAP+L-BA group)and baicalin high-dose group(APAP+H-BA group).Pathological tissue sections was made by H&E staining,liver pathological changes were observed,serum ALT and AST levels were detected,liver GSH,GSH-Px and MDA levels were measured by kit,and protein expression levels of Bax and Bcl-2 were tested by Western blot.The test results showed that compared with the CON group,ALT increased,GSH and GSH-Px decreased,MDA level increased,Bax expression increased and Bcl-2 decreased in the APAP group,and the H&E staining results showed significant liver damage.Compared with APAP group,ALT,AST,MDA and Bax levels in L-BA and H-BA groups were decreased,while GSH,Gsh-PX and Bcl-2 levels were increased.H&E staining showed that liver injury was significantly alleviated.In conclusion,BA had a protective effect on APAP-induced acute liver injury in mice,and its mechanism might be related to alleviating the imbalance of free radical-antioxidant oxidase caused by oxidative stress in mice liver and regulating the expression of apoptosis-related proteins Bax and Bcl-2.
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