Effects of Baicalin on Hepatic Oxidative Stress and Apoptosis Induced by Acetaminophen in Mice
To explore the baicalin(BA)on acute liver injury induced by acetaminoPhen(APAP)protection,and preliminarily discuss its mechanism,the mice were randomly divided into blank control group(CON group),baicalin control group(BA group),APAP model control group(APAP group),baicalin low-dose group(APAP+L-BA group)and baicalin high-dose group(APAP+H-BA group).Pathological tissue sections was made by H&E staining,liver pathological changes were observed,serum ALT and AST levels were detected,liver GSH,GSH-Px and MDA levels were measured by kit,and protein expression levels of Bax and Bcl-2 were tested by Western blot.The test results showed that compared with the CON group,ALT increased,GSH and GSH-Px decreased,MDA level increased,Bax expression increased and Bcl-2 decreased in the APAP group,and the H&E staining results showed significant liver damage.Compared with APAP group,ALT,AST,MDA and Bax levels in L-BA and H-BA groups were decreased,while GSH,Gsh-PX and Bcl-2 levels were increased.H&E staining showed that liver injury was significantly alleviated.In conclusion,BA had a protective effect on APAP-induced acute liver injury in mice,and its mechanism might be related to alleviating the imbalance of free radical-antioxidant oxidase caused by oxidative stress in mice liver and regulating the expression of apoptosis-related proteins Bax and Bcl-2.
万方数据
维普
