In order to improve the economic benefits of British red kidney beans and develop functional products with high added value,the nutritional value and particle size distribution of British red kidney bean protein antioxidant peptide component(BRKBPAPC)were analyzed,and the changes of antioxidant activity during the digestion of BRKBPAPC were evaluated by in vitro simulation digestion method.The results showed that BRKBPAPC contained 8 essential amino acids,and the content of amino acids related to antioxidant activity was higher.The amino acid ratio coefficient(SRCAA)of BRKBPAPC was 66.01,the essential amino acid index(EAAI)was 86.13,and the biological price(BV)was 82.18.The essential amino acid composition of BRKBPAPC was in line with the FAO/WHO standard model,and it was a good plant-derived polypeptide.BRKBPAPC has a uniform particle size distribution and only one peak,between 400 and 800 nm.The results of in vitro digestion simulation showed that the antioxidant activity and stability of BRKBPAPC were significantly improved compared with non-ultrafiltration peptides(P<0.05);The highest scavenging rate of BRKBPAPC was 81.83%for DPPH free radical,82.76%for hydroxyl free radical and 48.84%for ABTS free radical.The study provided a theoretical basis for the development of red kidney bean products in the UK.
关键词
英国红芸豆/抗氧化肽/体外模拟消化/氨基酸组成/抗氧化活性
Key words
British red kidney bean/antioxidant peptide/in vitro simulated digestion/amino acid composition/antioxidant activity
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