一株木质素降解细菌的分离、鉴定与降解条件优化

Isolation,Identification and Optimization of Degradation Conditions of Lignin Degrading Strain

张亚莉 ¹王敬红 ²洪艳华 ³晏磊 ¹章圣龙 ⁴崔宗均 ⁵王伟东⁶

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作者信息

1. 农业农村部东北平原农业绿色低碳重点实验室/黑龙江八一农垦大学生命科学技术学院,大庆 163319
2. 农业农村部东北平原农业绿色低碳重点实验室/黑龙江八一农垦大学生命科学技术学院,大庆 163319;黑龙江八一农垦大学农学院
3. 黑龙江八一农垦大学农学院
4. 黑龙江国宏节能环保有限公司
5. 中国农业大学农学院
6. 农业农村部东北平原农业绿色低碳重点实验室/黑龙江八一农垦大学生命科学技术学院,大庆 163319;东北林业大学生命科学学院;黑龙江八一农垦大学农学院
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摘要

高效降解和转化木质素的微生物资源对于秸秆类生物质资源化利用至关重要.通过梯度稀释和苯胺蓝脱色法从木质素降解复合菌系中筛选出木质素降解细菌,经 16S rRNA和生理生化试验将菌株归类为Pseudomonas guguanensis.采用单因素、Plackett-Burman和响应面分析的方法确定菌株最佳培养条件.单因素试验表明:木质素添加量、装液量、pH为显著影响因子;响应面试验确定最佳培养条件:装液量为 28 mL·100 mL-1、木质素添加量为 0.6 g·L-1、pH为 7.4.在此条件下,木质素降解率为 29.1%,比优化前提高了 16.4%,研究丰富了降解木质素的微生物资源.

Abstract

Microbial resources that can efficiently degrade and transform lignin are very important for the utilization of straw biomass resources.The lignin degrading bacteria were screened from the lignin degrading complex strains by gradient dilution and aniline blue decolorization.The strain was classified as Pseudomonas guguanensis by 16S rRNA and physiological and biochemical tests.Single factor,Plackett-Burman and response surface analysis were used to determine the optimal culture conditions.Single factor test showed that lignin addition,liquid loading and pH were significant factors.The optimal culture conditions were determined by response surface test:liquid loading was 28 mL·100 mL-1,lignin addition was 0.6 g·L-1 and pH was 7.4.Under this condition,the degradation rate of lignin was 29.1%,which was 16.4%higher than that before optimization.It enriched the microbial resources for lignin degradation.

关键词

木质素/细菌/降解条件优化/假单胞菌

Key words

lignin/bacteria/optimization of degradation conditions/Pseudomonas

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基金项目

国家自然科学基金(U22A20444)

国家自然科学基金(32170132)

黑龙江省揭榜挂帅科技攻关项目(2022ZXJ08B01)

黑龙江八一农垦大学三纵计划重点项目(ZDZX202105)

出版年

2024

黑龙江八一农垦大学学报

黑龙江八一农垦大学

黑龙江八一农垦大学学报

影响因子：0.888

ISSN：1002-2090

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