Isolation,Identification and Optimization of Degradation Conditions of Lignin Degrading Strain
Microbial resources that can efficiently degrade and transform lignin are very important for the utilization of straw biomass resources.The lignin degrading bacteria were screened from the lignin degrading complex strains by gradient dilution and aniline blue decolorization.The strain was classified as Pseudomonas guguanensis by 16S rRNA and physiological and biochemical tests.Single factor,Plackett-Burman and response surface analysis were used to determine the optimal culture conditions.Single factor test showed that lignin addition,liquid loading and pH were significant factors.The optimal culture conditions were determined by response surface test:liquid loading was 28 mL·100 mL-1,lignin addition was 0.6 g·L-1 and pH was 7.4.Under this condition,the degradation rate of lignin was 29.1%,which was 16.4%higher than that before optimization.It enriched the microbial resources for lignin degradation.
ligninbacteriaoptimization of degradation conditionsPseudomonas
万方数据
维普
