PrxⅠRegulates Apoptosis of Triple Negative Breast Cancer Cells by Mediating ROS and Mitochondrial Damage
It aimed to elucidate the killing effect of Erastin on triple negative breast cancer cells and the related regulatory mechanism of Prx Ⅰ on triple negative breast cancer MDA-MB-231 cells.The effects of knock-down Prx Ⅰ on ROS,mitochondrial damage and cell death in MDA-MB-231 cells were detected by immunofluorescence,Western Blot and MTT,respectively.The results showed that knockdown Prx Ⅰ could induce a large amount of ROS production and mitochondrial damage in the cell.At the same time,the expression level of autophagy related protein ERK was significantly increased after Prx Ⅰ knock-down,indicating that autophagy was activated by ERK after Prx Ⅰ knock-down.Finally,the regulation of MDA-MB-231 cell death in human triple-negative breast cancer is described,which provided a new idea for the treatment of triple-negative breast cancer and a theoretical basis for the clinical application of Prx Ⅰ preparation.
Triple negative breast cancerPrxⅠROSmitochondrial damageautophagy