It aimed to elucidate the killing effect of Erastin on triple negative breast cancer cells and the related regulatory mechanism of Prx Ⅰ on triple negative breast cancer MDA-MB-231 cells.The effects of knock-down Prx Ⅰ on ROS,mitochondrial damage and cell death in MDA-MB-231 cells were detected by immunofluorescence,Western Blot and MTT,respectively.The results showed that knockdown Prx Ⅰ could induce a large amount of ROS production and mitochondrial damage in the cell.At the same time,the expression level of autophagy related protein ERK was significantly increased after Prx Ⅰ knock-down,indicating that autophagy was activated by ERK after Prx Ⅰ knock-down.Finally,the regulation of MDA-MB-231 cell death in human triple-negative breast cancer is described,which provided a new idea for the treatment of triple-negative breast cancer and a theoretical basis for the clinical application of Prx Ⅰ preparation.
关键词
三阴性乳腺癌/PrxⅠ/活性氧/线粒体损伤/自噬
Key words
Triple negative breast cancer/PrxⅠ/ROS/mitochondrial damage/autophagy
维普
万方数据