黑龙江八一农垦大学学报2024,Vol.36Issue(5) :15-23.DOI:10.3969/j.issn.1002-2090.2024.05.003

马铃薯种质资源抗黑痣病鉴定及SSR聚类分析

Identification and SSR Cluster Analysis of Potato Germplasm Resources Resistant to Black Scurf

王紫雯 金光辉 王腾 王鹏程 高睿 吴国强
黑龙江八一农垦大学学报2024,Vol.36Issue(5) :15-23.DOI:10.3969/j.issn.1002-2090.2024.05.003

马铃薯种质资源抗黑痣病鉴定及SSR聚类分析

Identification and SSR Cluster Analysis of Potato Germplasm Resources Resistant to Black Scurf

王紫雯 1金光辉 1王腾 1王鹏程 1高睿 1吴国强1
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作者信息

  • 1. 黑龙江八一农垦大学农学院,大庆 163319
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摘要

马铃薯黑痣病已成为影响马铃薯产量和商品价值的重要土传病害.为丰富马铃薯抗黑痣病种质资源,提高抗黑痣病育种工作效率,研究通过54 份马铃薯种质资源地下茎与匍匐茎黑痣病抗性评价,共筛选出 7 份中抗资源,分别为克 17-33-270、龙18-124-8、龙 18-7-100、ND11-7-22、克 18-134-5、克 17-115-1、克 17-6-9.研究利用 8 对SSR适宜引物对 7 份抗性材料DNA进行PCR扩增,获得SSR多态性条带 42 条,多态性比率 89.36%,遗传相似性系数在 0.444 4~0.833 3 之间,以相似性系数 0.62 为基准,可将 7份抗性材料分为 3类,其中克 18-134-5 和克 17-115-1 为一类;龙 18-124-8 和龙 18-7-100 为一类;克17-33-270、ND11-7-22 和克17-6-9 为一类.

Abstract

Potato black scurf has become an important soil-borne disease affecting potato yield and commodity value.In order to enrich potato germplasm resources resistant to black scurf and improve the efficiency of breeding for resistance to black scurf,54 potato germplasm resources were evaluated for resistance to black scurf in underground stems and stolons.A total of 7 moderately resistant resources were screened,which were Ke 17-33-270,Long 18-124-8,Long 18-7-100,ND11-7-22,Ke 18-134-5,Ke 17-115-1 and Ke 17-6-9.Eight pairs of SSR suitable primers were used to amplify the DNA of seven resistant materials by PCR,and 42 polymorphic bands were obtained,the polymorphism rate was 89.36%,and the genetic similarity coefficient was between 0.444 4 and 0.833 3.Based on the similarity coefficient of 0.62,the seven resistant materials could be divided into three categories,including ke 18-134-5 and Ke17-115-1.Long 18-124-8 and Long 18-7-100were in the same category;Ke17-33-270,ND11-7-22 and Ke17-6-9 were in the same category.

关键词

马铃薯(Solanum/tuberosum/L.)/种质资源/抗性鉴定/黑痣病/SSR分子标记

Key words

potato(Solanum tuberosum L.)/genetic resources/resistance identification/black scurf/SSR molecular markers

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基金项目

2022年黑龙江省"揭榜挂帅"科技攻关项目()

出版年

2024
黑龙江八一农垦大学学报
黑龙江八一农垦大学

黑龙江八一农垦大学学报

影响因子:0.888
ISSN:1002-2090
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