Effect of Activation and Inhibition of GPR120 on LTA-induced Expression of Pro-inflammatory Genes and Cell Viability in Mammary Epithelial Cells of Dairy Cows
To investigate the effect of the GPR120 gene on the mitigation of LTA-mediated inflammation in mammary epithelial cells of dairy cows,Mac-T cells were treated with GPR120 activator TUG891 and inhibitor AH7614,and Mac-T cells were stimulated with 20 μg·mL-1 of LTA to construct a mammary inflammatory cell model.The mRNA expression of the relevant intracellular pro-inflammatory factors and their effects on cell viability were examined by qRT-PCR and CCK-8 assay in the Mac-T cell inflammatory response.The results showed that the mRNA expression levels of GPR120 were significantly up-regulated and inhibited by the treatment of GPR120 activator and inhibitor for 3 h.The mRNA expression levels of pro-inflammatory cytokines TNF-α,IL-1β and IL-6 were significantly up-regulated by the stimulation of Mac-T cells with 20 μg·mL-1 LTA for 24 h(P＜0.05).The expression levels of the pro-inflammatory cytokines TNF-α,IL-1β and IL-6 were significantly up-regulated(P＜0.05),indicating that the Mac-T cell model of mastitis in cows constructed using LTA was successful,and that LTA stimulation significantly promoted the expression level of GPR120(P＜0.05);activation of GPR120 significantly alleviated the release of the inflammatory cytokines TNF-α,IL-6 and IL-1β induced by LTA(P＜0.05);and inhibition of GPR120 significantly increased the expression level of GPR120(P＜0.05).further significantly exacerbated the high expression of pro-inflammatory cytokines TNF-α,IL-1β and IL-6 produced by Mac-T cells induced by LTA(P＜0.01).Within 48 h of stimulation of Mac-T cells with LTA,cell viability was significantly reduced(P＜0.01),while treatment with activator significantly alleviated the reduction in cell viability induced by LTA(P＜0.01),which was fur-ther triggered by treatment with inhibitor.It suggested that GPR120 was involved in the LTA-induced inflammatory response in bovine mammary epithelial cells and that activation of GPR120 slowed down the inflammatory response,which provided an important basis for screening for mastitis resistance genes in cows.
万方数据
维普
