Effects of Exosome miR-126-5P on Lipopolysaccharide-induced Inflammation in Bovine Mammary Epithelial Cells
Exosomes are a type of extracellular vesicle with a diameter of 30-200 nm secreted by various types of cells,which can play the regulatory functions through a variety of miRNAs.In order to clarify the regulatory effect of exosomal miR-126-5P on LPS-induced inflammation in dairy cow mammary epithelial cells,TargetScan and miRanda software were used to predict the target gene of miR-126-5P.Then,the cell models of miRNA overexpression and inhibition were constructed by liposome transfection technique,and the regulation of miR-126-5p on inflammation was further verified by q-PCR and Western blotting methods.The results showed that miR-126-5p was down-regulated 0.3-fold in the LPS-stimulated cell-derived exosome.Target prediction results showed that TNF Superfamily Member 13b(TNFSF13B),C-C Motif Chemokine Ligand 22(CCL22),Immunoglobulin Superfamily Member 11(IGSF11),Interleukin 5 Receptor Subunit Alpha(IL5RA),Interleukin 13(IL13)might be the main target genes of miR-126-5P.After treatment of Mac-T cells with the miR-126-5p mimic,the phosphorylation level of I-κBα and the level of IL-6 mRNA were up-regulated.After treatment of Mac-T cells with miR-126-5P inhibitor,the phosphorylation level of I-κBα and the level of IL-6 mRNA were down-regulated.The results laid a foundation for further exploring the inflammatory regulation mechanism of exosome miRNA derived from dairy cow mammary epithelial cells.
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