Study on Optimization of Purification Process and Identification of the Structure of Hordenin
Using barley malt as raw material,the purification process was optimized to obtain barley malt base and its structure was identified.Orthogonal experiments were carried out to optimize the extraction process of hordenine by ultrasonic and microwave collaborative extraction method.Response surface optimization analysis was used to explore the optimal process conditions for purification of hordenine by macroporous resin,and the structure was identified by HPLC mass spectrometry.The optimal extraction process was liquid to solid ratio of 45∶1,temperature of 60℃,microwave time of 40 min,microwave power of 400 W,extraction content of 2.74 mg·g-1.Under this condition,the yield was 82.64%.The optimal purification process was the adsorption flow rate of 193.00 BV·h-1,pH 6.21,elution flow rate of 1.57 mL·min-1 and elution alcohol concentration of 84.60%.The extract by mass spectrometry was 90%pure hordenine.It provided a theoretical basis for the mechanism research and product development of barley malt.
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