The proteins and salts in the mixed droplets interact to produce crystallization during the droplet evaporation and lead to some unique deposition patterns at the end of the droplet evaporation.Droplets containing particles exhibit several drying stages to form the final pattern on the substrate.The study observes the deposition patterns formed by the evaporation of mixed droplets of proteins(BSA,OVA)and KCl at different concentrations through a microscope,and uses the gray level co-occurrence matrix(GLCM)to obtain the eigenvalues of the deposition morphology.Experimental results show that different types of proteins will lead to changes in deposition morphology.The reason is speculated to be the structural differences between the two proteins.The eigenvalues changes significantly in the KCl concentration range of1～2 wt%.In addition,the edge ring deposition trend is close to linear with increasing protein concentration.Such structural features can help identify different proteins and characterize their concentrations.
关键词
蛋白质/结晶/蒸发/咖啡环效应
Key words
Protein/Crystallization/Evaporation/Coffee ring effect
维普
万方数据