CCL4与BDL致大鼠肝损伤中α-SMA、Hab18G/CD147表达的差异
CCL4 and BDL Induced Liver Injury in Rats on the Difference of the Expression of α-SMA and Hab18G/CD147
位小杰 1薛超 2史文哲 3张聚丹2
作者信息
- 1. 郑州工业应用技术学院医学院,郑州 451150
- 2. 佳木斯大学基础医学院,黑龙江佳木斯 154007
- 3. 佳木斯大学医学部医学人文教研室,黑龙江佳木斯 154007
- 折叠
摘要
探究CCL4腹腔注射和胆总管结扎(Bile Duct Ligation,BDL)致大鼠肝损伤中α-SMA、Hab18G/CD147表达的差异,为法医学鉴定药物性肝损伤提供科学参考.选取雄性SD大鼠60只,随机分为CCL4对照组(N)、BDL对照组(S)、CCL4肝损伤组(C)、BDL肝损伤组(B),每组各15只.各组分别在实验1 w、3 w、5 w时断颈处死5只大鼠,采血检测谷丙转氨酶(Glutamic pyruvic transami-nase,ALT)、谷草转氨酶(Aspertate aminotransferase,AST),总胆红素(Total bilirubin,TBIL)、直接胆红素(Direct bilirubin,DBIL),检测α-SMA、Hab18G/CD147mRNA 的表达情况.qRT-PCR 检测结果显示,C 组 α-SMAmRNA、Hab18G/CD147mRNA 的表达量在 1 w、3 w、5 w同时间段相比均高于B组(P＜0.05).与对照组相比较,C组、B组血清中ALT、AST、DBIL、TBIL含量均增加,说明大鼠肝脏均有不同程度的损伤;CCL4与BDL肝损伤有不同特点,在肝损伤过程中α-SMA、Hab18G/CD147mRNA的表达随着损伤时间的延长表达量逐渐增加.
Abstract
The study investigates the effects of intraperitoneal injection of CCL4 Bile Duct Ligation(BDL)on the differential expression of α-SMA and Hab18G/CD147.A total of 60 male SD rats were randomly divided into CCL4 control group(N),BDL control group(S),CCL4 liver injury group(C),and BDL liver injury group(B),with 15 rats in each group.5 rats in each group were euthanized by neck transection in 1,3,and 5 weeks of the experiment.Blood samples were collected to detect Alanine aminotransferase(ALT),Aspartate aminotransferase(AST),Total bilirubin(TBIL),and Direct bilirubin(DBIL).Expression of α-SMA and Hab18G/CD147mRNA was tested.The qRT PCR test showed that the expression levels of α-SMA mriRNA and Hab18G/CD147mRNA of Group C were higher than those of Group B in 1 w,3 w,and 5 w(P＜0.05).Compared with the control group,the serum levels of ALT,AST,DBIL,and TBIL in Group C and Group B increased,indicating varying degrees of liver damage.CCL4 and BDL have different characteristics in liver injury,and in the process of liver injury the expression of α-SMA and Hab18G/CD147mRNA gradually increases with the prolongation of injury time.
关键词
大鼠肝损伤/CCL4/BDL/α-SMA/Hab18G/CD147Key words
Liver injury/CCL4/BDL/α-SMA/Hab18G/CD147引用本文复制引用
基金项目
黑龙江省2023年大学生创新创业训练计划项目(S202310222102)
河南省医学教育研究项目(WJLX2023153)
出版年
2024
NETL
NSTL
万方数据