In order to improve the quality of biology teaching in high school,the study integrates the project-based teaching method into the teaching of biology in high schools,so as to cultivate students'innovative consciousness,thinking ability and problem-solving ability.The study conducts questionnaire survey method,and uses SPSS 19.0 statistical software for data analysis.The results show that the teaching effect of project-based biology is above the average level,mainly reflecting in teaching background evaluation,teaching input evaluation,teaching process evaluation and teaching result evaluation,but the teaching result evaluation mechanism needs to be improved.From the perspective of the second-level indicators,although some achievements have been gained in students'ability training,it still needs to be improved.There are no significant difference in the effects of project-based teaching in senior biology teaching,teaching background evaluation,teaching input evaluation,teaching process evaluation and teaching result evaluation,but there are significant differences in student achievements(P>0.05).It is suggested to strengthen the construction of project-based biology result evaluation mechanism,and the cultivation of students'ability,so as to improve the teaching effect and students'learning ability of biology.