Since Chinese medicinal materials are easily contaminated by fungi such as Aspergillus flavus during transportation and storage,the study analyzes the inhibitory effects of three different antibacterial methods of essential oil on Aspergillus flavus.The effects of clove,litsea cubeba and oregano essential oils on the inhibition of Aspergillus fllavus mycelium at different concentrations are investigated by K-B,fumigation and oil resistance plate method.The results show that Aspergillus flavus is highly sensitive to oregano essential oil.Oregano essential oil completely inhibits the growth of Aspergillus fllavus at the concentration of 0.1 μL/mL,which can be used to develop Aspergillus flavus growth inhibitors in cereals,food and traditional Chinese medicine.