The study systematically optimizes the fermentation process of Bacillus Polymyxoides from the main aspects of medium composition(carbon source,nitrogen source,calcium carbonate)and fermentation conditions(pH value,inoculation amount,liquid loading amount,rotational speed and temperature),etc.;and finds out the optimal fermentation conditions through triangle bottle test and 100 L fermenter amplification test,so as to improve the bacteria quantity and spore rate of Bacillus Polymyxoides.The results show that the optimal fermentation medium is 2％soybean meal meal+2％sucrose+1％corn starch+0.6％ammonium sulfate,and the optimal calcium carbonate addition is 0.5％.Under the conditions of 37 ℃,200 r/min,60 L/min ventilation and initial pH value of 7.2,the fermentation in 100 L fermenter for 32 h achieves the best bacterial content and spore rate.This verifies the feasibility and stability of the experimental conditions.
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