In order to further develop and use Cirsium setosum ,the effect of 2 ,4‐D ,6‐BA and NAA for Cirsium setosum callus induction were discussed by single factor and orthogonal test .The results showed that under sin‐gle factor ,2 ,4‐D optimal concentration range from 0 .8 to 1 .0 mg?L‐1 ,6‐BA range from 0 .8 to 1 .0 mg?L‐1 and NAA range from 2 .4 to 2 .6 mg?L‐1 .Orthogonal test results showed that the MS + 2 ,4‐D 1 .0 mg?L‐1 + 6‐ BA 1 .0 mg?L‐1 + NAA 2 .4 mg?L‐1 medium induced best callus .The three kinds of hormones all could promote Cirsium setosum stem callus formation and growth .