Gene Cloning,Interacting Protein Screening and Stress Resistance Analysis of AmArg1 Gene from Amygdalus mira Koehne
In order to investigate the arginase 1(AmArg1)of Amygdalus mira,the full-length ORF of the arginase 1,AmArg1 was cloned and therefore,the deduced protein sequence was predicted for structure and physicochemical properties via bioinformatics analysis.The results showed that the protein was an acidic,non-secreted protein with no signal peptide structure.AmArg1 was transferred into yeast cells and it was non-toxic and non-self-activating for yeast cells.The interacting proteins,AmLhcb1 and AmRabB1b of AmArg1 were screened by yeast two hybrid assays,and their interactions were validated by BiFC analysis.Transgenic yeast in response to NaCl,mannitol,and CuSO4 stress was preliminarily explored,and found that over-expression of AmArg1 might increase the tolerance to these stresses.
Amygdalus mira Koehneinteracting proteinsabiotic stressAmArg1 gene
万方数据
维普
