Effects of Solid-State Fermentation Time of Different Bacterial Strains on Content and Antioxidant Properties of Licorice Flavonoids
In order to fully explore and utilize licorice resources and promote the antioxidant research of fermented licorice,three different probiotics(yeast,Aspergillus oryzae,and Aspergillus niger)were selected as the research objects to ferment flavonoids in licorice.Ethanol reflux method was used to extract flavonoids from licorice,and single factor experiments were conducted to optimize the fermentation time under the fixed conditions of inoculation volume of 10％,fermentation temperature of 28 ℃,water content of 80％,and 30 g of licorice.Based on the optimal fermentation time,the crude extract of licorice flavonoids was purified using D101 macroporous adsorption resin and its relatedin vitro antioxidant capacity was determined.The effect of solid-state fermentation time of different bacterial strains on the content and antioxidant properties of licorice flavonoids was explored.The results showed that the content of flavonoids was highest in yeast fermentation for 5 days,Aspergillus oryzae fermentation for 5 days,and Aspergillus niger fermentation for 3 days.Therefore,based on this,purification was carried out using macroporous resin,and the flavonoid content increased after purification.Aspergillus niger increased from 63.8 mg·g-1 to 90.6 mg·g-1,Aspergillus oryzae increased from 78.2 mg·g-1 to 101.8 mg·g-1,yeast increased from 54.9 mg·g-1 to 82.6 mg·g-1,and unfermented increased from 39.6 mg·g-1 to 57.9 mg·g-1;The scavenging ability of different strains of fermented licorice on DPPH and ABTS free radicals was significantly higher than that of unfermented licorice.In summary,there is a significant difference in the flavonoid content of licorice before and after fermentation.After purification with macroporous adsorption resin,the flavonoid content of licorice increases significantly,and licorice has better antioxidant activity after fermentation.
Licorice flavonoidssolid state fermentationmacroporous resinantioxidant activityDPPHABTS
万方数据
维普
