Identification of a Pathogen Causing Root Rot Disease in Sorghum
In order to identify the pathogen species of a separated sorghum root rot,the dipping root inoculation method was used to verify Koch's postulates,and the fungus was identified based on the analysis of ITS andβ-tubulin gene sequences,combined with morphological characteristics.The results indicated that the colony of strain ZY5 was velvety on the front,regular and circular,with a yellow brown color.The reverse side of the colony was dark yellow brown to black.Chlamydospores were either solitary or in chains,and sized 8-17 μm× 10-12 μm.Conidia were oval-shaped,aseptate,and sized 2.0-3.0 μm × 1.0-1.5 μm.The phylogenetic tree constructed based on β-tubulin sequences grouped ZY5 closely with Epicoccum sorghinum,with a support rate of 98％.Based on morphological characteristics and the constructed phylogenetic tree,strain ZY5 was identified as Epicoccum sorghinum.This is the first report domestically and internationally of Epicoccum sorghinum causing sorghum root rot.
万方数据
维普
