Application of Different Sampling Methods and Detection Techniques in the Pathogenetic Diagnosis of Severe Pneumonia
Objective:The qualification rate of specimens from different sampling methods and the positive rate of results from different microbial detection techniques varied,and the application of different sampling methods and detection techniques in the pathogenetic diagnosis of severe pneumonia was explored with a view to improving the accuracy of pathogenetic diagnosis.Methods:The clinical data of 123 patients with severe pneumonia admitted to the hospital from January 2018 to December 2020 were retrospectively analyzed.Data on patients'sputum culture by transnasal or tracheal intubation,alveolar lavage fluid culture,and alveolar lavage fluid macro-genomic second-generation sequencing technology(mNGS)pathogen detection were also collected,and specimens were analyzed for pass rates and positive rates.Results:Of the 123 patients,70% of the sputum specimens retained by transnasal or tracheal intubation were qualified,41(33.3%)had pathogenic bacterial growth,92%of the alveolar lavage specimens were qualified,76(61.8%)had pathogenic bacterial growth,and 107(86.9%)had nucleic acid fragments of pathogenic bacteria detected in mNGS of alveolar lavage.After treatment with anti-infection,anti-shock,and organ support,115 patients were cured and discharged,and 8 died.Conclusion:Alveolar lavage mNGS can increase the positive rate of pathogen diagnosis in patients with severe pneumonia,which may provide a basis for clinical treatment.
Severe pneumoniaAlveolar lavage fluidMacrogenomic second-generation sequencing technologySputum culture
万方数据
维普
