Network Pharmacologic Analysis of Liver Protection Tablets Combined with Antisense Granules in the Treatment of EBV Liver Injury
Objective:To investigate the molecular mechanism of liver protection tablets combined with anti-sense granules for the treatment of Epstein-Barr virus(EBV)liver injury by network pharmacology combined with molecular docking technique.Methods:The TCMSP,HERB database and Swiss Target Prediction platform were utilized to screen and predict the active ingredients and potential targets of Liver Protecting Tablets combined with Anti-Sense Granules.The GeneCards database was used to search for genes related to EBV liver injury.Cytoscape 3.9.1 software was utilized to construct the active ingredient-target network of the drug and screen the key ingredients.The potential targets of liver protection tablets combined with anti-sense granules for the treatment of EBV liver injury were analyzed by GO and KEGG pathway enrichment,and imported into the STRING platform to construct a PPI network,and the core targets were screened using the MCC and MNC algorithms.Finally,CB-Dock was utilized to perform molecular docking between key components and core targets.Results:A total of 96 active ingredients of liver protecting tablets combined with anti-sense granules were collected,and 57 targets of action of liver protecting tablets combined with anti-sense granules for the treatment of EBV liver injury were identified.The results of GO and KEGG pathway enrichment analysis were mainly related to cytokine receptor binding,cytokine activity,and human cytomegalovirus infection,etc.Molecular docking results confirmed that the core targets STAT3,AKT1,JUN,and TP53 could stably bind to the core components quercetin,kaempferol,luteolin,acacetin,and isorhamnetin.Conclusion:Liver protection tablets combined with anti-sense granules mainly act on the core targets of STAT3,AKT1,JUN and TP53 through the key components of quercetin,kaempferol,lignocerotoxin,chrysin and isorhynchophyll,which are involved in the regulation of cytokine-related biological cellular processes,and ultimately play a role in the treatment of EBV liver injury.
万方数据
维普
