Effects of Long Non-coding RNA SNHG1 on Proliferation and Migration of Colorectal Cancer and its Regulatory Mechanism
Objective:To investigate the effects of long non-coding RNA SNHG1 on Proliferation and migration of colorectal cancer and its regulatory mechanisms.Methods:Real-time fluorescence quantitative PCR was used to detect the expression level of IncRNA SNHG1 in human normal colorectal ePithelial cells(FHC)and colorectal adenocarcinoma cell line(HT29)cells.HT29 cells were divided into interference group(si-SNHG1)and control group(si-NC)and transfected with SNHG1 siRNA and NC siRNA,respectively.MTT was used to detect cell proliferation viability,transwell assay was used to detect cell migration ability,and Western blot was used to detect the exPression of proteins related to JAK-STAT signaling pathway.Results:The expression of SNHG1 in HT29 cells was significantly higher than that in FHC cells,and the difference was statistically significant(t=17.045,P＜0.05).Compared with the control group,cell value-added activity and the number of migrating cells were reduced in the interference group,and the difference was statistically significant(t=16.317,12.714,P＜0.05).The difference in STAT3 protein expression was not statistically significant(t=1.063,P＞0.05).P-STAT3 Protein expression was reduced,and the difference was statistically significant(t=15.473,P＜0.05).Conclusion:SNHG1 was highly expressed in CRC cells and could promote the proliferation and migration of CRC cells.The mechanism may be related to the upregulation of P-STAT3 protein expression by SNHG1 to promote the activation of JAK-STAT signaling pathway.
Long non-coding RNASNHG1Colorectal cancerProliferationMigration
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