Association of DACT2 Promoter Methylation with Cervical Cancer Cell Invasion and Metastasis in Cervical Cancer Tissues
Objective:To investigate the correlation between DACT2 promoter methylation and cervical cancer cell invasion and metastasis in cervical cancer tissues.Methods:60 cases of cervical cancer patients in the hospital from January 2020 to March 2022 were selected as the observation group,and then 60 cases of healthy medical examination in the same period were selected as the control group.Semi-quantitative reverse transcription polymerase chain reaction(RT-PCR)and real-time fluorescence quantitative RT-PCR were used to detect the expression of DACT2 mRNA,and immunohistochemistry of DACT2 was observed.DACT methylation levels were analyzed by methylation-specific polymerase chain reaction(MSP)and bisulfite genome sequencing(BGS).Pearson's test was used to analyze the association between DACT2 promoter methylation and cervical cancer cell invasion and metastasis.Results:There was a statistically significant difference in the pathological characteristics of patients with cervical cancer in terms of stage,presence or absence of cellular metastasis,and the percentage of patients with depth of cervical infiltration when compared(t=4.747,5.368,0.834;P＜0.05),while there was no statistically significant difference when comparing the age and the size of the tumors(t=1.004,0.895;P＞0.05).The incidence of DACT2 demethylation in cervical cancer tissues,paracancerous tissues and normal tissues were 45.00%,6.67%and 5.00%,respectively,with statistically significant differences(x2=26.454,P＜0.05).The DACT2 mRNA and protein expression levels in the methylated group of cervical cancer were significantly lower than those in the non-methylated group of cervical cancer,and the difference was statistically significant(t= 11.332,8.543;P＜0.05).The results of correlation analysis showed that DACT2 promoter methylation was a factor that triggered the invasion and metastasis of cervical cancer cells,and the difference was statistically significant(P＜0.05).Conclusion:The main factor influencing the invasion and metastasis of cervical cancer cells is DACT2 promoter methylation,and the higher the rate of DACT2 promoter methylation,the higher the degree of cell invasion and metastasis.Therefore,clinical monitoring of patients'DACT2 promoter methylation should be closely monitored to help optimize the treatment plan for cervical cancer patients.
Cervical cancer tissueDACT2 promoter methylationInvasion and metastasis of cervical cancer cellsRelevance
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