Animal Model of Systemic Inflammatory Response Syndrome in Rats Prepared with Yeast Polysaccharide
Objective:To prepare for the next step of exploring the pathogenesis of SIRS and subsequent intervention,the experiment was conducted to establish a stable rat model of systemic inflammatory response syndrome(SIRS)by investigating the use of yeast polysaccharide.Methods:Wistar rats were all injected intraperitoneally with yeast polysaccharide-paraffin suspension to prepare the SIRS animal model,and the model group rats were divided into 4 groups of 400 mg/kg,500 mg/kg,600 mg/kg and 700 mg/kg according to different doses.The general condition,temperature,WBC,of rats 24h after administration and test index:TNF-α,IL-6 and pathological HE staining of liver and lung organs were evaluated.Results:Rats injected intraperitoneally with yeast polysaccharide-paraffin suspension at the doses of 600 mg/kg and 700 mg/kg met the criteria of SIRS animal model,and the survival rate of rats in the 600 mg/kg group was 40％at 48 h,which had a long survival cycle,while the mortality rate of rats in the 700 mg/kg group could reach 50％after 24 h in the short term.Conclusion:Intraperitoneal injection of yeast polysaccharide-paraffin suspension is a successful method to prepare more stable SIRS model rats,and the rats in the 600 mg/kg dose group can survive for a longer period of time.So it can be used to study the pathogenesis of SIRS and also prepare for the subsequent intervention experiments of SIRS.
万方数据
维普
