Application Analysis of Bacterial Culture Method and Gram Staining Method in the Detection of Vaginal Bacteria
Objective:To compare the application value of bacterial culture method and Gram staining method in vaginal bacterial examination.Methods:120 suspected patients with bacterial vaginitis were studied.From October 2021 to October 2022,all patients were examined by bacterial culture and Gram staining.The positive rates of vaginal bacteria in two different ways were compared.Amsel's clinical diagnostic standard was taken as the gold standard.The diagnostic value of the two ways and the detection rates of different pathogens were compared.The time and satisfaction of the two groups were compared.Results:The sensitivity,specificity,accuracy,positive predictive value and negative predictive value of bacteria culture in the diagnosis of vaginal bacteria were 95.65％,82.14％,92.50％,94.62％and 85.19％respectively.The sensitivity,specificity,accuracy,positive predictive value and negative predictive value of Gram staining in the diagnosis of vaginal bacteria were 83.70％,50.00％,75.83％,84.62％and 48.28％respectively.There was difference in comparison(P＜0.05).The detection rates of Gartner,Corynebacterium and Enterococcus by bacterial culture method were significantly higher than those by Gram staining method(x2=6.628,4.694,7.336;P＜0.05).The examination time of bacterial culture method was(4.21±1.18)h,which was longer than that of Gram staining method(0.44±0.08)h(t=34.918,P＜0.05).The patient satisfaction was higher than that of Gram staining method,and there was difference between groups(Z=2.422;P＜0.05).Conclusion:Compared with Gram staining method,bacterial culture method can accurately diagnose different types of vaginal bacteria,with strong sensitivity and specificity,and patients are more satisfied.However,the former is short in time,can quickly obtain the diagnosis results,and can reasonably choose the test method according to the clinical needs.
万方数据
维普
