Laboratory Assays Combined with Cytomorphometric Analysis in MA with MDS Value in the Differential Diagnosis
Objective:To analyze the laboratory test indexes,and peripheral blood and bone marrow cells to differential diagnose megaloblocytic anemia(MA)and myelodysplastic syndrome(MDS).Methods:From October 2020 to June 2022,21 MDS and 17 MA patients were studied,tested for blood routine and VitB12,made peripheral blood smear and bone marrow smear,and observed cell morphological characteristics.Results:Pancytopenia,RBC,Hb and MCV between MA and MDS(P＞0.05),while VitB12 and MCV were＞100 fL(P＜0.05).Peripheral blood cell morphology analysis showed that there were differences in polychromatic erythrocytes,carbring,central light area expansion,primitive granulocytes,binuclear granulocytes,granulocytes,false Pelger-Huet malformation and neutrophil nuclear segmentation,with statistically significant difference(x2=14.491,14.568,10.238,12.533,14.568,7.495,4.661;P＜0.05).There were no statistically significant difference in large platelets and abnormal platelets(P＞0.05).Bone marrow cell morphology analysis showed Erythroid binucleus,odd nuclei,macroprotoluene,macroprotoluene,macromesoprotoluene,macroerythroid,macrored cells,and mature red blood cells were of uneven size,granulocyte(≥5％),macromesopluene,macrolatepluene,macrorod nucleus,pseudopelger-huet deformity,unbalanced karyoplasmic development,many neutrophil nuclear lobes(＞5 lobes),reduced or no granules,megakaryonuclear lobulated,small megakaryon,double round megakaryon,multi-round megakaryon,small megakaryon,and the differences were statistically significant(x2=8.3 14,4.259,7.112,20.761,18.087,9.401,15.641,21.478,9.547,8.197,5.768,3.619,4.259,4.661;P＞0.05).Conclusion:Both MA and MDS blood images have different degrees of pancytopenia.MA red blood cell morphology is dominated by large cells(MCV＞100 fL),and VitB12 is significantly reduced,combined with serum VitB12 determination to facilitate the identification of the two.MA pathological hematopoiesis mainly reflects the giant age of three lines.MDS has some clonal evidence,manifested in the erythroid dikaryres,primitive granulocytes,double nuclear granulocytes,false Pelger-Huet malformation,micro megakaryus,micro megakaryus,and multiround megakaryocytes,etc.
万方数据
维普
