Analysis of the Value of Microcolumn Gel Method in the Detection of Hemolytic Disease of Newborn
Objective:To analyze the clinical value of microcolumn gel method in detection of hemolytic disease of newborn.Methods:142 cases of suspected hemolytic disease of newborn in the hospital were tested by tube method and micro-column gel method.Results:The blood types of 142 cases were Type A(45 cases),type B(46 cases)and type O(51 cases),and 31 cases were diagnosed.Among them,21 cases were type A(21 cases),10 cases were type B(10 cases)and 0 case were type O(0 case).In direct anti-human immunoglobulin test,free antibody test and antibody release test,the positive rate of blood group A was the highest,which was higher than that of blood group B(x2=4.785,5.683,4.874,5.463;P＜0.05).There was no significant difference among the other groups(P＞0.05).Among the diagnosed cases,5 cases were positive for hemolytic test,1 case was positive for direct anti-human immunoglobulin test and free antibody test,the results of direct anti-human immunoglobulin test and antibody release test were both positive in 3 neonates.The results of free antibody test and antibody release test were both positive in 4 neonates.The results of antibody release test in 18 neonates were positive.The detection rate of blood group antigen by microcolumn gel method was 100％,which was higher than that by tube method 98.59％(x2=4.783,P＜0.05).The positive rates of the three hemolytic tests were 7.04％,7.75％and 38.73％respectively,which were higher than 4.93％,4.23％and 14.79％of the test tube method(x2=5.683,11.783,4.781;P＜0.05).Conclusion:The clinical value of microcolumn gel method in the detection of hemolytic disease of newborn is significant due to its simple operation,great specificity,high sensitivity and accuracy.
Microcolumn gel methodHemolytic disease of newbornDetection value
万方数据
维普
